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. 2007 Jan 15;8(3):195–211. doi: 10.1111/j.1600-0854.2006.00524.x

Figure 5: Intracellular complexes containing Gag and ABCE1 increase to approximately the size of capsids over time.

Figure 5:

COS-1 cells expressing HIV-1 were subjected to pulse radiolabeling for 15 min followed by chase with unlabeled medium. At indicated times, cells were harvested and subjected to velocity sedimentation, fractionation and immunoprecipitation under native conditions with αABCE1. A) p55Gag associated with ABCE1 in fractions representing ∼10S ∼80/150S and ≥500S complexes was quantitated and shown as assembly profiles. Positions of ∼10S ∼80/150S and ≥500S complexes are indicated. B) Amount of p55Gag associated with ABCE1 in fractions representing ∼80/150S and ≥500S complexes at indicated time-points was quantitated and graphed. All data are from one experiment and are representative of four independent experiments.