Figure 3. CalDAG-GEFI deficiency causes impaired β₁ integrin– and β₂ integrin–mediated adhesion of neutrophils in vitro.

(A and B) Neutrophil adhesion to fibronectin (A) or fibrinogen (B) in vitro. WT or CalDAG-GEFI–deficient neutrophils isolated from bone marrow were added to fibronectin- or fibrinogen-coated plates and incubated for 30 minutes in the presence or absence (i.e., resting) of 300 nM LTB₄ or 3 or 30 nM PAF. Plates were washed and adherent neutrophils were counted. n = 4. *P < 0.05, **P < 0.01, ***P < 0.001.