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(A) Surface expression and recombinant human BAFF (rhBAFF) binding of WT and mutant mTACI in transfected 293T cells. (B) snIP analysis of the association of WT and mutant mTACI in ER microsomes. Asterisk (lane 6) represents mixing control in which TACI-SBP, TACI-PC, and TACI-YFP monomers were translated separately and mixed prior to solubilization and IP. (C) FRET analysis of the association of WT and mutant mTACI in 293T cells.
