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. 2007 Feb 8;35(5):1569–1577. doi: 10.1093/nar/gkl1159

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Protein kinase activity of the multiprotein complex. (A) Phosphorylated polypeptides were revealed upon incubation of the Mono S fraction in the presence of [γ-32P] ATP alone (lane 1), or in combination with histone H1 (lane 2), histone plus p21 (lane 3) or histone plus Olomucine (lane 4). (B) The Mono S fraction was immunoprecipitated with anti-cyclin A antibodies and the immunoprecipitated material was probed with antibodies against cyclin A (lane 1) or anti-phosphoSer/Thr (lane 2).