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. 2007 Feb 18;35(5):1649–1659. doi: 10.1093/nar/gkm046

Figure 2.

Figure 2.

Reverse transcription initiation of HIV-1 leader RNA transcripts with variable 3′ ends. (A) DNA (left) or tRNAlys3 (right) primers were annealed to HIV-1 transcripts. Primers were extended by addition of 32P-CTP and HIV-1 RT enzyme. Extended primers were separated on a denaturing acrylamide gel. The 1/2 tRNA signal represents a half tRNA species that is discussed in detail in the context of Figure 8. (B) Relative reverse transcription initiation efficiency with tRNAlys3 as primer was calculated by comparison with the DNA-primed signal. The relative efficiency of reverse transcription initiation (RTI) of the 1–368 template was set at 1. Quantification and standard deviations of four independent experiments are shown.