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The secondary structure model of part of the HIV-1 leader RNA and the tRNAlys3 primer are shown. Mutant 2L has a 7-nt substitution in the PAS motif and mutant 2R has a substitution on the opposite side of the base-paired PAS domain. The 2L and 2R mutations were combined in the 2LR mutant. The asterisks at position 37–39 of the tRNA mark the position of a cleavage product that is referred to as 1/2 tRNAlys3 in Figure 8.
