Figure 6.
Reverse transcription initiation on the 3′-nested set of wt, 2L, 2R and 2LR transcripts. (A) DNA or tRNAlys3 primers were annealed to the indicated transcripts. Primers were extended by HIV-1 RT with 32P-CTP and separated on denaturing acrylamide gels. Gel fragments of the DNA- and tRNA-primed extensions are shown. (B) Relative reverse transcription initiation efficiency of the different transcripts. Initiation of the wt368 template was set at 1. The average and standard deviation of four independent experiments was quantified.