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DNA-, RNA- and tRNA-primed reverse transcription. (A) The 3 primers were tested on the 1–368 transcripts of wt and mutants 2L, 2R and 2LR. Primers were extended with 32P-CTP by HIV-1 RT. Extended primers were separated on a denaturing acrylamide gel. Indicated are cDNA products of DNA, RNA, tRNA and 1/2-tRNA primers. (B) Quantified data of three experiments. The efficiency of DNA-primed reverse transcription initiation of wt is set at 1.
