Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cap guanine-N2 methylation is not essential in S. pombe. Total RNA from tgs1+ and tgs1Δ strains of S. pombe was subjected to immunoprecipitation with anti-TMG antibody bound to protein A sepharose beads. Aliquots of input RNA (I), immunoprecipitated RNA (P) and unbound RNA from the supernatant (S) were analyzed by northern blotting using probes to detect U1, U2, U4 and U5 snRNA and the uncapped 5.8S RNA as described in methods section. Autoradiograms of the blots are shown.
