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. 2007 Feb 8;35(5):1589–1600. doi: 10.1093/nar/gkl1170

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Purification and activity of I-TevIII. (A) Purification. Lane 1, size markers; lane 2, crude extract; lane 3, soluble lysate; lane 4, 0.2% PEI supernatant; lane 5, 45% saturated (NH₄)₂SO₄; lanes 6–8, NaCl gradient peak. (B) Cleavage assay. For each metal assayed, two amounts of protein were utilized, 0.2 µg or 0.4 µg. Added metals were at a concentration of 0.1 mM. Cleavage products are marked with a P and substrate is marked with an S. Cleavage in the presence of NiSO₄ is not shown. The spurious band in lanes 2 and 5 are of unknown origin.