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. 2007 Feb 8;35(5):1589–1600. doi: 10.1093/nar/gkl1170

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Cleavage and DNA-binding assays of alanine-substitution mutants. (A) Schematic representation of I-TevIII indicating mutated amino acids. The numbers represent amino acid positions that were changed to alanine. ZF = zinc finger with the consensus sequence. (B) H–N–H mutants. For each mutant assayed, two levels of protein were utilized, 0.2 µg or 0.4 µg. Cleavage and DNA-binding assays are shown. Samples were separated on native 8% polyacrylamide gels. Cleavage products are marked with a P, and unbound DNA with an S. (C) Zinc finger mutants. Analysis and labeling as in panel A. Asterisks represent protease-treated samples.