Figure 3.

Ribonuclease H degradation of various hybrid duplexes. An 18-nt 5′-³²P-labeled target RNA (5′-ACG UGA AAA AAA AUG UCA-3′) was preincubated with complementary 18-nt I–V, and then added to reaction assays containing either (A) E. coli RNase HI or (B) human RNase HII (110 nM assay shown here). Aliquots were removed as listed in the figure (in minutes). Base sequences of antisense oligomers are given in Table 1. See the Results and Discussion section for detailed assay conditions. Gels from human RNase H assay at lower enzyme concentration and shorter exposure times are available as Supplementary Data.