Table 1.

Antisense oligonucleotide sequences and thermal denaturation studies^a

Sequence							Tm (RNA target)	Tm (DNA target)
I	5′-UGA	CAU	ttt	ttt	UCA	CGU-3′	60.0	51.0
II	5′-UGA	CAU	TTT	TTT	UCA	CGU-3′	51.0	36.0
III	5′-UGA	CAU	TTT	TTT	UCA	CGU-3′	62.0	50.1
IV	5′-tga	cat	ttt	ttt	tca	cgt-3′	42.1	55.5
V	5′-UGA	CAU	UUU	UUU	UCA	CGU-3′	59.1	40.2

^aLegend: RNA, DNA, 4′S-FANA, 2′F-ANA. Complementary strands were as follows: RNA, 5′-ACG UGA AAA AAA AUG UCA-3′; DNA, 5′-acg tga aaa aaa atg tca-3′.