Table 2.
siRNA sequences and thermal denaturation studiesa
| Duplex | Tm (°C) | IC50 (nM) | |
|---|---|---|---|
| Ctl | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 62.3 | 0.16 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| Ctl-p | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | n.d. | 0.04 |
| 3′-ggCGAACUUCAGAAAUUAAUUp-5′ | |||
| T1 | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 60.2 | 0.10 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| F1 | 5′-GCUUGAAGUCUUUAATTAAtt-3′ | 63.0 | 0.20 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| T2 | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 57.2 | 0.17 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| F2 | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 60.0 | 0.31 |
| 3′-ggCGAACUTCAGAAAUUAAUU-5′ | |||
| T3 | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 62.0 | 3.6 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| T3p | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | n.d. | 0.16 |
| 3′-ggCGAACUUCAGAAAUUAAUUp-5′ | |||
| F3 | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 62.1 | 1.0 |
| 3′-ggCGAACUUCAGAAAUUAATT-5′ | |||
| F3p | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | n.d. | 0.04 |
| 3′-ggCGAACUUCAGAAAUUAATTp-5′ | |||
aLegend: RNA, DNA, 4′S-FANA, 2′F-ANA. Sense strands are listed on top and antisense strands below. Duplexes with names ending in ‘p’ were 5′-phosphorylated on the antisense strand (see text for details).