Table 3.
Effect of significantly modified sense strands with FAU point modifications in the antisense stranda
| Duplex | Tm (°C) | IC50 (nM) | |
|---|---|---|---|
| Ctl | 5′-GCUUGAAGUCUUUAAUUAAtt-3′ | 62.1 | 0.16 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| Ctl-gg | 5′-GCUUGAAGUCUUUAAUUAAgg-3′ | 61.7 | 0.87 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| Ctl-f | 5′-GCTTGAAGTCTTTAATTAAGG-3′ | 66.0 | 7.8 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| Ctl-fr | 5′-GCTTGAAGTCTTTAATTAATT-3′ | 63.0 | 2.4 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| Ctl-fm | 5′-GCTTGAAGTCTTTATTAAA3′ | 62.1 | >20 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| T2-f | 5′-GCTTGAAGTCTTTAATTAAGG-3′ | 61.0 | 17 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| T2-fr | 5′-GCTTGAAGTCTTTAATTAATT-3′ | 56.6 | 0.60 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| T2-fm | 5′-GCTTGAAGTCTTTATTAAA-3′ | 54.1 | >20 |
| 3′-ggCGAACUUCAGAAAUUAAUU-5′ | |||
| F2-f | 5′-GCTTGAAGTCTTTAATTAAGG-3′ | 65.6 | 10.8 |
| 3′-ggCGAACUTCAGAAAUUAAUU-5′ | |||
| F2-fr | 5′-GCTTGAAGTCTTTAATTAATT-3′ | 61.6 | 0.87 |
| 3′-ggCGAACUTCAGAAAUUAAUU-5′ | |||
| F2-fm | 5′-GCTTGAAGTCTTTATTAAA-3′ | 61.1 | >20 |
| 3′-ggCGAACUTCAGAAAUUAAUU-5′ | |||
aLegend: RNA, DNA, 4′S-FANA, 2′F-ANA. Mismatches are indicated with gray text. Duplexes with names ending in ‘f’ contained fully 2′F-ANA sense strands, ‘fr’ indicated a 2′F-ANA sense strand containing five RNA inserts near the sense 3′-end, and ‘fm’ indicated a 2′F-ANA sense strand with two internal mismatches near the sense 3′-end (see text for details).