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. 2007 Jan 30;35(5):e32. doi: 10.1093/nar/gkl1171

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Histograms from flow cytometric sortings of optimized fluorescence clones (Opt) from ExLib1 and ExLib2. (A) Overlay plots from analyses of the original ExLib1 library (filled histogram) and re-cultured subpopulations of the libraries (gray) from after the first (left) and second (right) sorting round for highly fluorescent cells using 488 nm fluorescence gate values of >800 (gate 1) and >2000 (gate 2), respectively, (B) Overlay plots from analyses of the original ExLib2 library (filled histogram) and re-cultured subpopulations of the libraries (gray) from after the first (left) and second (right) sorting round for highly fluorescent cells using 488 nm fluorescence gate values of >1000 (gate 1) and >4000 (gate 2), respectively; (C) Overlay plots from analyses of the individual clones from the ExLib1 library (gray histograms) and the reference clone pBR-TrpL-ZEGFP (black histogram); (D) Overlay plots from analyses of the individual clones from the ExLib2 library (gray histograms) and the reference clone pBR-TrpL-ZEGFP (black histogram). The pre-amplifier gain was set to 600 V (A and B) or 500 V (C and D).