Table 1.
Listing of some characteristics for a selection of clones from ExLib1 and ExLib2a
| Clone ID | Sequence (5′–3′)b | Startc | RelPCd | RelFe |
|---|---|---|---|---|
| ExLib1-Low8 | ATGAAAGCGATCT TCGTGCTGAAG | nd | ||
| ExLib1-Low9 | ATGAAGGCCATAT TCGTGCTCAAA | nd | ||
| ExLib1-Low2 | TTGAAGGCAATAT TCGTCCTCAAA | nd | ||
| ExLib1-High3 | ATGAAAGCAATAT TCGTATTAAAG | nd | ||
| ExLib1-High2 | ATGAAAGCTATTT TTGTACTCAAG | nd | ||
| ExLib1-High1 | ATGAAAGCCATCT TCGTGTTAAAG | nd | ||
| ExLib1-Opt9 | ATGAAAGCAATAT TCGTACTCAAG | 1:st | 1.1 | 4.3 |
| ExLib1-Opt10 | ATGAAAGCAATCT TTGTCTTGAAA | 1:st | 1.3 | 4.0 |
| ExLib1-Opt3 | ATGAAAGCAATAT TCGTGTTGAAG | 1:st | 1.3 | 3.0 |
| ExLib2-Low4 | ATGGTGTGGGGTA GGGAGCATCAG | nd | ||
| ExLib2-Low1 | TTGGGGGGTACGC GGGGTCAGGCT | nd | ||
| ExLib2-Low7 | ATGGCGGCTACGT CGAAGCCGGTG | nd | ||
| ExLib2-High3 | ATGAAGAATAGGT CGACGCAGCAG | 1:st | 1.3 | 2.7 |
| ExLib2-High1 | ATGTTTAAGGGGG GGGAGGGGGTT | 2:nd | ||
| ExLib2-High5 | ATGTTGGCGGCGA TTGAGGGGAAG | 2:nd | ||
| ExLib2-Opt7 | ATGGTGGATGGTC TGAAGAGGGGG | 2:nd | 2.7 | 15.8 |
| ExLib2-Opt1 | ATGAGTGATCCTA GTAGGAGGGGG | 2:nd | 1.6 | 5.5 |
| ExLib2-Opt4 | ATGAGTAGTCAGG GGTTGAGGAGT | 2:nd | 1.0 | 4.7 |
| ExLib2-Opt5 | ATGACGTAGCATC TGAATAAGGAG | nd | ||
| ExLib2-Opt6 | ATGTAGGTGAAGA TGGGGGAGGTT | nd | ||
| ExLib2-Opt10 | ATGGGTAGGGCCG TGAGGAGGAG | nd | ||
| ExLib2-Opt9 | ATGCGGGAGCGTG AGACGGGGGAG | nd | ||
| ExLib2-Opt3 | ATGAAGACGTCGC GGGGGGAGTAG | nd | ||
| ExLib2-Opt8 | TTGGCGAAGGGGA AGTTGATGATG | nd | ||
| ExLib2-Opt2 | TTGAATTGGAGGA AGGTGAGGGAG | nd |
aClones within each group are listed according to their mean fluorescence intensity values as measured by flow cytometry (first = highest fluorescence).
bSequences of the 24 nucleotide windows subjected to the variegation. Putative SD sequences are indicated in bold for clones of which purified protein products have been analyzed by mass spectrometry. The sequence giving the highest number of continuous bases complementary to the CCUCC core of the E. coli anti-SD sequence ACCUCCUUA is shown (36).
cThe nomenclature 1:st and 2:nd, refers to a translational start at the first start codon (A/T)TG or the second start codon TTG discussed in the text (Figure 1).
dRelPC is the relative plasmid copy number for a given clone (i.e. the average number of library plasmid copies/chromosome in a clone compared to the corresponding value for the pBR-TrpL-ZEGFP reference clone) (mean values from triplicate experiments) as determined in the materials and methods section.
eRelF is the relative fluorescence intensity for a given clone (i.e. the fluorescence intensity value for a clone compared to the fluorescence intensity value of the pBR-TrpL-ZEGFP reference clone) (mean value from triplicate experiments), determined by flow cytometry analyses as described in the materials and methods section.