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. 2007 Feb 7;35(5):1555–1568. doi: 10.1093/nar/gkm022

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — NMR spectra of the G6*G7 (A–C) and G6G7* (D–F) duplexes. (A) G6*G7 imino proton spectrum (12.0–14.0 ppm) in H₂O buffer solution at 5°C. The imino proton assignments are also shown. (B) G6*G7 expanded NOESY (250 ms mixing time) contour plot in H₂O buffer solution at 5°C. The NOE connectivities between imino protons of all guanines and amino protons of cytosines across the G · C base pairs and thymidine imino to adenine H2 protons in a T · A base pair. Cross-peaks A–H are assigned as follow: A1: G6*(H1)–C19(NH₂ non-bonded H atom); A2: G6*(H1)–C19(NH₂-bonded H); A3: G6*(H1)–G6*(NH₂); B1: G20(H1)–C5(NH₂-non-bonded H); B2: G20(H1)–C5(NH₂-bonded H); C1: G7(H1)–C18(NH₂-non-bonded H); C2: G7(H1)–C18(NH₂-bonded H); D: BP(H8)–G6*(H1); E: T3(H3)–A22(H2); F: T23(H3)–A2(H2); G: T14(H3)–A11(H2); H: T10(H3)–A15(H2). (C) G6*G7 NOE 250 ms mixing time contour 2D plot showing distance connectivities between imino-to-imino protons in the duplex. The labels are, I: G13(H1)–T14(H3); J: G4(H1)–T3(H3); K: G6*(H1)–G7(H1); L: G4(H1)–G20(H1); M: T3(H3)–T23(H3); N: G16(H1)–T10(H3). (D) G6G7* Imino proton spectrum (12.0–14.0 ppm) in H₂O buffer solution at 5°C. (E) G6G7* expanded NOESY (250 ms mixing time) 2D contour plot in H₂O buffer solution at 5°C. The NOE connectivities between imino protons of all guanines and amino protons of cytosines across the G · C base pairs and thymidine imino to adenine H2 protons in T · A base pairs are shown. Cross-peaks A–H are assigned as follows: A1: G7*(NH)–C18(NH₂ non-bonded H); A2: G7*(NH)–C18(NH₂-bonded H); A3: G7*(NH)–G7*(NH₂); B1: G6(NH)–C19(NH₂ non-bonded H); B2: G6(NH)–C19(NH₂-bonded H); C1: G17(NH)–C8(NH₂ non-bonded H); C2: G17(NH)–C8 (NH₂-bonded H); D: BP(H8)–G7*(H1); E: T3(H3)–A22(H2); F: T14(H3)–A11(H2); G: T23(H3)–A2(H2); H: T8(H3)–A15(H2). (F) G6G7* NOE 250 ms mixing time 2D contour plot showing distance connectivities between imino-to-imino protons in the G6G7* duplex. Peak labels I–N are defined as I: T3(H3)–T23(H3); J: G16(H1)–T10(H3); K: G4(H1)–T3(H3); L: G6(H1)–G20(H1); M: G16(H1)–G17(H1); N: G4(H1)–G20(H1).