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Base substitutions in the single-stranded regions of domains 1–2 of the PSIV IRES to identify uridine residues that crosslink to the 40S ribosomal proteins. Mutations indicated by A–I correspond to the mutant IRES elements used in the experiments in Figure 5. To facilitate T7 transcription and HindIII digestion, the 5′ and 3′ terminal sequences were modified from the native sequence shown in Figure 1.
