Figure 7.

Macromolecular tandem mass spectrometry reveals topology of Kid–Kis–parD DNA region I complexes. Tandem mass spectrometry was performed on Kid–Kis and Kid–Kis–parD DNA in ammonium acetate (50 mM), pH 5.8. (A) Tandem mass spectra of Kis₂–Kid₂–Kid₂–Kis₂ after selection of the 20⁺ ion and (B) tandem mass spectra of Kis₂–Kid₂–Kid₂–Kis₂–parD DNA complex after selection of the 21⁺ ion. Acceleration voltages varied between 25 and 65 V. Kid and Kis are indicated with blue rectangles and orange ellipses, respectively and the parD DNA fragment with double strand. Each complex is represented by an appropriate combination of rectangles, ellipses and/or DNA double strand.