Table 2.
Accuracy and precision of the LC-MS/MS method for the determination of genomic DNA methylation in in vitro methylated PCR product and genomic DNA extracted from cell lines
| Sample type | Intra-day (Ave. ± S.D.) | Inter-day (Ave. ± S.D.) | |||
|---|---|---|---|---|---|
| 5mdC/2dG 5mdC/[2dC + 5mdC] (Te) | C.V.% | Accuracy% | 5mdC/2dG 5mdC/[2dC + 5mdC] (T) | C.V.% | |
| PCa | NDc | NAd | NA | NA | NA |
| MPCb | 26.2 ± 2.4 | 9.16 | 105.2 | NA | NA |
| 28.8 ± 0.49 (29.1) | 1.69 | 98.8 | |||
| Kasumi-1 | 4.87 ± 0.24 | 5.02 | NA | 5.36 ± 0.70 | 1.30 |
| 5.82 ± 0.21 (5.72) | 3.69 | 5.94 ± 0.14 (6.30) | 2.37 | ||
| K562 | 2.49 ± 0.10 | 4.08 | NA | 3.03 ± 0.34 | 11.3 |
| 2.91 ± 0.11 (2.93) | 3.63 | 3.19 ± 0.28 (3.56) | 8.74 | ||
aPC, PCR product of C-myc promoter; bMPC, Fully methylated PCR product of C-myc promoter; cND, Not detected; dNA, not available; eT, Theoretical