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. 2007 Feb 8;35(5):1578–1588. doi: 10.1093/nar/gkm011

Figure 8.

Figure 8.

Toeprint caused by L20 binding to the B. subtilis infC leader mRNA. Oligonucleotide HP697 was extended on a wild type and ‘C’ mutant (CC41,42 → GG, see Figure 5) in vitro transcript of the infC leader region. Where indicated the transcript was incubated with the B. subtilis r-proteins L20 or L17 prior to the extension reaction. The reverse transcriptase stop caused by L20 bound to the transcript is indicated by an arrow; it corresponds to a primer extension arrest at uridine residue 72 (see Figure 6). The sequence ladder was generated with the same primer HP697, the encircled nucleotide indicates the arrest position on the cDNA.