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. 2007 Apr 19;3(5):263–273. doi: 10.7150/ijbs.3.263

Fig 5.

Fig 5

The ERK phosphorylation induced by PMA is mediated by PKC. A, The pre-incubation with PKC specific inhibitor, 5 μM GF102903X, totally abolished the ERK1/2 phosphorylation induced by 300 nM PMA in HEK 293 transfected with Ng wild type. B, Western blot analysis of ERK1/2 phosphorylation after 300 nM PMA treatment in HEK 293 transfected with Ng wild type, S36A and I33Q mutants. The time points after the treatment of PMA were shown above.