Figure 6.

Effect of CLAMP on the cellular metabolism of HDL-CE taken up via SR-BI. CHO cells and CHO-CLAMP cells were transiently transfected with either SR-BI or SR-BIΔC15 and incubated at 37°C for 4 h with [¹²⁵I/³H-CE]HDL. The efficiency of conversion of HDL-CE into UC was determined by dividing the amount of cellular [³H]UC by the amount of cellular [³H]CE as described in Materials and Methods. Before calculating the ratios, the specific values for each construct were corrected by subtracting the specific background values from cells transfected with the control plasmid pcDNA3. Values represent means ± SEM (n = 3).