Table 2.

Effect of CLAMP on the uptake efficiency by SR-BI

Cell	Transfected gene	125I-HDL cell association (a), ng HDL protein/mg cell protein	[3H]CE selective uptake (b), ng HDL protein/mg cell protein	Uptake efficiency index*
CHO-K1
	Control	38	152
	SR-BI	150	745	100
	SR-BIΔC15	139	661	95
CHO-CLAMP
	Control	34	166
	SR-BI	348	1,567	85
	SR-BIΔC15	153	682	82

^*Uptake efficiency index was calculated as described (12), i.e., in CHO-K1 cells = [(b − 152)/(a − 38)] × (100/5.29); for wild-type SR-BI, a = 150, b = 745, and (b − 152)/(a − 38) = 5.29; and in CHO-CLAMP cells = [(b − 166)/(a − 34)] × (100/5.29).