Table 4.
Viral loads in tissues from IT-inoculated cynomolgus macaques, day 8 post-inoculationa
| Virus | Macaque no. | Plaque assay (log10 PFU/g tissue or mL CSF) |
RT-PCR (log10 copies/g tissue or mL CSF) |
||||
|---|---|---|---|---|---|---|---|
| Frontal cortexb | Occipital lobeb | CSFb | Frontal cortex | Occipital lobe | CSF | ||
| wt VSVIN | F22190F | 6.74 | –c | 1.74 | 9.10 | 5.78 | 5.76 |
| F21545M | 2.16 | – | – | 6.61 | – | – | |
| F22212M | 2.87 | – | – | 8.40 | 5.05 | – | |
| F16739F | 5.67 | – | – | 8.64 | 5.01 | – | |
| rVSVIN | F21392F | 5.21 | – | – | 6.66 | – | – |
a
Viral loads were assayed for macaques in all groups; all of those that survived to day 21 had undetectable viral loads in both assays.
b
Tissues collected during necropsy, CSF collected immediately prior to necropsy.
c
“–” indicates below the limit of detection of the assay (plaque assay = 2 log10 PFU/g of tissue and 1 log10 PFU/mL of CSF; RT-PCR assay = 5 log10 copies/g of tissue and 5.3 log10 copies/mL of CSF).