Table 1.
Influence of proteins and nucleotides on the reactivation yields of CS*
| αB-crystallin
|
GroEL (11)
|
||
|---|---|---|---|
| Added component | Reactivation yield, % | Added component | Reactivation yield, % |
| None | 1 | None | 1 |
| ATP | 1 | ATP | 3 |
| αB | 14 | GroEL | 0 |
| GroEL + ATP | 16 | ||
| αB + ATP | 25 | GroEL + GroES + ATP | 28 |
| αB + ADP | 14 | GroEL + ADP | ND |
| αB + ATPγS | 17 | GroEL + ATPγS | ND |
| αB + AMP-PCP | 17 | GroEL + AMP-PCP | ND |
| αB + AMP-PNP | 9 | GroEL + AMP-PNP | ND |
| BSA + ATP | 3 | BSA + ATP | 7 |
| Lysozyme + ATP | ND | Lysozyme + ATP | 6 |
| γd-crystallin + ATP | 1 | γd-crystallin + ATP | ND |
| Alcohol dehydrogenase + ATP | 4 | Alcohol dehydrogenase + ATP | ND |
| Maltose-binding protein + ATP | 5 | Maltose-binding protein + ATP | ND |
*
Refolding and reactivation of CS in the presence of αB-crystallin was performed as described in Methods. The effects of various proteins and nucleotides on the reactivation of chemically denatured CS are shown as percent reactivation at t = 60 min for equimolar ratios of protein-to-CS. Results from refolding and reactivation of CS in the presence of GroEL are taken from Buchner et al. (11).