Table 1.
Sequences of primers used for the constructs and the LightCycler
| Primers | Sequences |
| α5-helix deletion | |
| Fragment 1-F | 5' CGGAATTCATGGATGATCCGAGAGGATCTG 3' |
| Fragment 2-F | 5' GCTCTAGATCACTGATTTTCGTCTTCCAC 3' |
| Fragment 1-R | 5' CGCCTTGGACTCGCCAGTGTTAGCTGC 3' |
| Fragment 2-R | 5' GCCCAAGGCCACAGGAAACGACAGGAAGG 3' |
| PCR of ARS307 | |
| ARS307-F | 5' ATATTGCAATTACTTCTTCTCATGCAC 3' |
| ARS307-R | 5' ATATTGCAATTACTTCTTCTCATGCAC 3' |
| PARS307-F | 5' GGTAGGGATAATAATCTGTAATGAGGA 3' |
| PARS307-R | 5' TATGTCGACGAGTGAGTTGGA 3' |
| Real-Time PCR | |
| RT-ARS307-F | 5' GCAGTAGCACATGGACACATTCAC 3' |
| RT-ARS307-R | 5' AGGGTTTCTGGAGAGGTAGGGATA 3' |
| NegARS307-F | 5' ATTTTGTGGTGGATTGGAG 3' |
| NegARS307-R | 5' GCTTGTTTGTGCAACTAAACG 3' |
| ARS1-F | 5' GAAAATAGGTTATTACTGAGTAG 3' |
| ARS1-R | 5' CCTGCGATGTATATTTTCCTG 3' |
| R2.5-F | 5' CATCAATTGTGCACTCGGAC 3' |
| R2.5-R | 5' GAACACGGCAATTGTAGGTGG 3' |