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. 2007 May 16;2(5):e450. doi: 10.1371/journal.pone.0000450

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A, Gene targeting strategy. Open box, R26 exon 1; DT-A, diphtheria toxin expression cassette. SA, splice acceptor; X, XbaI. B, Southern analysis of ES cell clones. Genomic DNAs were digested with XbaI and detected by 5′ external or 3′ internal probes. C, PCR genotyping of R26^floxneoWnt4; Col2a1-Cre mice. Primers shown in panel A (arrowheads) amplify R26 wild-type (∼600-bp) and targeted (∼350-bp) alleles. Cre-specific primers yield a ∼550-bp band to identify mice carrying the Col2a1-Cre transgene.