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Representative miRNAs in Chlamydomonas. (A–C) Predicted fold-back structures of selected miRNA precursors. Sequences corresponding to the mature miRNAs are shown in red, and sequences corresponding to the miRNA*s are shown in blue. Protruding stem–loops in the 3′ arm of the hairpins are indicated by slashes (\). (D) Confirmation of miRNA expression by Northern blot using end-labeled oligonucleotide probes as indicated. A synthetic 32P-labeled 21-nt RNA oligo was used as a size marker.
