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Reduced FoxA2+ and Nkx.2+, but not Olig2+ cell specification in E9.5 Gas1; Shh compound mutants. Forelimb-level sections of E9.5 wild-type (A–C), Gas1−/− (D–F), and Gas1−/−; Shh+/− (G–I) embryos were examined for FoxA2 (green; A,D,G), Nkx2.2 (red; B,E,H), and Olig2 (C,F,I) expression. Bar: A, 50 μm.
