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. 2007 May 15;21(10):1244–1257. doi: 10.1101/gad.1543607

Figure 8.

Figure 8.

Gas1; Cdo compound mutants display severely reduced Shh signaling. (A–G) Nasal process defects in E10.5 Gas1; Cdo embryos are shown. Brackets indicate the distance between nasal pits. (H) A Shh−/− E10.5 embryo is shown for comparison. Examination of Nkx2.2 (red) and Olig2 (green) expression in E10.5 Gas1; Cdo (I–O) and Shh−/− (P) forelimb-level sections. Forelimb-level expression of FoxA2 (red; Q–X) and Shh (green; Y–Z,AA–FF) in E10.5 Gas1; Cdo and Shh−/− embryos. In situ hybridization analysis of the notochord marker CAIII in E9.5 Gas1; Cdo embryos (GG–MM). Discontinuous CAIII expression is detected in a Shh−/− E9.5 embryo (NN), indicative of notochord degeneration. Arrows in NN highlight the broken CAIII expression. Bars: A, 1 mm; I, 50 μm; GG, 1 mm. For Gas1−/−; Cdo−/− embryos, a total of five embryos were examined with similar results.