Figure 7.

Time-dependent decrease in IRP1 mRNA levels after exposure to IFN-γ/LPS. RAW 264.7 cells were activated with 10 units/ml IFN-γ and 50 ng/ml LPS for 1–8 h. Total RNA was extracted and assayed for IRP1 and NOS2 mRNA expression by Northern blotting, as described in Materials and Methods. Blots were hybridized sequentially with IRP1 (Top), NOS2 (Middle), and GAPDH (Bottom) ³²P-labeled cDNA probes. Data are representative of four independent experiments.