Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of endogenous and exogenous NO on IRP1 mRNA levels. (A) RAW 264.7 cells were cultured for 16 h with 10 units/ml IFN-γ and 50 ng/ml LPS, in the presence or absence of 1 mM l-NMMA. Nitrite released was measured in the culture medium. Total RNA was extracted and IRP1 mRNA levels were analyzed by Northern blotting with a 32P-labeled IRP1 cDNA probe. (B) Cells were treated with 100 μM Sper/NO for different times. IRP1 mRNA levels were analyzed as described in A.
