. Author manuscript; available in PMC: 2007 May 4.

Published in final edited form as: Biochemistry. 2006 Apr 25;45(16):5309–5318. doi: 10.1021/bi052586d

Table 2.

Pharmacological Analysis of the FLAG M2-hNK-1-(His)₆ Receptor Stably Expressed in CHO K1 Cells^a

	second messenger production EC₅₀ (nM)
second messenger pathway	substance P	NKA	propionyl[Met(O₂)¹¹]-SP(7–11)
cAMP (NK-1M)	11 ± 6	1000 ± 350	>5000
IP (NK-1m)	3.0 ± 0.5	2.2 ± 0.8	11 ± 7

Potencies of SP, NKA, and propionyl[Met(O₂)¹¹]SP(7–11) in stimulating the accumulation of cAMP or IP were measured on whole CHO cells. The NK-1M and NK-1m binding sites had previously been shown to be related to cAMP and IP accumulation, respectively.