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Reverse-phase high-performance liquid chromatography (RP-HPLC) chromatogram of completely trypsinolyzed HbII complex. ESI-MS peaks subjected to ESI-MS zoom scan and tandem MS analyses and their corresponding RP-HPLC fractions were indicated on this Figure. Charge-states of peaks (inferred based on the ESI-MS zoom scan data) were indicated as superscripts (see also Figure 3b inset).
