Figure 2. Imprinting Analysis of Human and Murine KLF14 .

(A) Imprinted expression of murine Klf14 is shown. Sequence analysis of genomic DNA and RT-PCR products from 15.5-dpc hybrid mice are shown in the left and right panels, respectively. Genomic sequencing results indicate the genotype for JF1 (G) at the polymorphism. RT-PCR sequencing results show the expression of the JF1 allele in all tissues where JF1 is the maternal allele (upper row in right panel) and expression of the BL6 allele in the reciprocal cross (lower row in right panel), indicating maternal expression.

(B) Imprinted expression of human KLF14. The first column of panels shows genomic sequencing electropherograms for three fetal samples (rows) heterozygous for a polymorphism in KLF14. The second column presents the genotype for the corresponding maternal samples (maternal DNA was not available for fetus number 62). The third column shows sequencing results of RT-PCR products indicating the monoallelic expression of various tissues, as indicated on the right of the column. Results from fetus number 66, which is informative for parental origin, indicate that KLF14 is maternally expressed. *, sequencing of tongue, stomach, eye, kidney, and intestine cDNA from fetus number 62 showed monoallelic expression.

(C) Maternal expression of human KLF14 in somatic cell hybrids. RT-PCR was performed for three independent maternal or paternal monochromosomal hybrid cell lines for human Chromosome 7. Results confirm the maternal expression of KLF14, as seen in (B). The expression of the paternally expressed MEST and mouse A9 cell line, which lacks human Chromosome 7, are also shown.