Figure 2. AKH-Dependent Storage Fat Mobilization Strictly Depends on AKHR, but Not on brummer Lipase Function.

(A and B) Organismal fat content (A) and cellular phenotype of fat-storage tissue visualized by Nile red staining of lipid storage droplets (B) show excessive fat storage in AKHR¹ mutants and in flies lacking AKH-positive neuroendocrine cells by reaper-induced apoptosis (AKH-ZD mutants; for details see Materials and Methods) compared to the AKHR^rev control. AKH-dependent depletion of fat storage (compare AKH induced vs. AKH uninduced control in [A] and [B]) is blocked in flies lacking AKHR function (compare AKHR¹ mutant AKH induced vs. AKHR¹ mutant AKH uninduced control in [A] and [B]). Scale bar represents 25 μm.

(C) AKH induction reduces fat storage in bmm mutants (compare bmm¹ AKH induced vs. bmm¹ AKH uninduced control).