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. Author manuscript; available in PMC: 2008 Mar 6.
Published in final edited form as: FEBS Lett. 2007 Jan 30;581(5):809–814. doi: 10.1016/j.febslet.2007.01.046

Figure 2. Murine adiponectin is an octadecamer.

Figure 2

A. HMW adiponectin from 3T3-L1 adipocytes and from calf serum were analyzed by SDS-PAGE/western blot under non-reducing conditions. Lane 1: purified HMW complex from 3T3-L1 adipocytes; Lane 2: molecular weight markers, (250, 150, 100, 75, 50 and 37 kDa); Lane 3: bovine HMW adiponectin. B. Murine HMW adiponectin was heated to various temperatures for 10 minutes in non-reducing loading buffer prior to analysis by SDS-PAGE/western blot. The number of subunits in each oligomer are indicated. C. The molecular weights for the intermediates formed at 90°C (open squares) were interpolated from a standard curve obtained using the octadecamer, hexamer, and trimer (closed circles) from conditioned media of 3T3-L1 adipocytes.