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Transcription start site mapping of the −35 mutant fixRp2 mRNA. Total RNA was purified from the following strains grown anaerobically: 2068 (mutant at the −35 region in the A−68-to-C fixRnifA promoter region), 2035 (mutant at the −35 region in wild-type fixRnifA promoter region), and 8085 (wild-type fixRnifA promoter). Transcripts p1 (mainly from the fixRp1 promoter), and p2 (from the fixRp2 promoter) are indicated.
