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In vivo genomic DMS footprinting analysis of the B. japonicum fixRnifA promoter region. Anaerobic cultures of strains 8085, 8091, and N8085 were grown for 3 days and incubated with rifampicin before the exposure to DMS. Primer extension pattern of the fixRnifA top (A) and bottom (B) strands is shown. Control DNA methylated in vitro in the absence of proteins is shown on the left. Protected positions are indicated with ○, whereas the hypermethylated position is indicated with •.
