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IFN-γ and IL-4 relative gene expression in PRV-stimulated PBMCs. The relative quantities of cytokine mRNA were determined by relative quantitative reverse transcriptase PCR using β-actin gene expression as the housekeeping gene. The mean relative quantities of cytokine mRNA ± standard deviations are shown. (A) Mean relative IFN-γ mRNA quantities. (B) Mean relative IL-4 mRNA quantities. *, P < 0.05, calculated with the nonparametric Mann-Whitney test; #, no survivor.
