Skip to main content
. 2007 Feb 14;14(4):335–341. doi: 10.1128/CVI.00155-06

FIG. 3.

FIG. 3.

Precipitation of 60-kDa heat shock proteins with polyclonal anti-CRP antibodies. Ouchterlony analysis of the interactions among Hsp60, Hsp65, native, and modified CRP and polyclonal anti-CRP antibody. Well B, 2 mg/ml native CRP diluted in K3PO4 buffer (pH 7.8); wells C and D, Hsp60 (1 mg/ml and 0.5 mg/ml, respectively); well E, mCRP (2 mg/ml); wells F and A, Hsp65 (1 mg/ml and 2 mg/ml, respectively). Central well, polyclonal anti-CRP antibody (DAKO) diluted 1:10 in K3PO4 buffer (pH 7.8). Experiments were performed with 1% agarose.