TABLE 1.
Generation and characterization of MAbs against DEN-1 by Western blotting, ELISA, and PRNT assay
| MAb | Western blot result
|
ELISA result
|
DEN-1 PRNT50 (μg/ml) | Specificitya | ||||
|---|---|---|---|---|---|---|---|---|
| DEN-1 | DEN-2 | DEN-3 | DEN-4 | DEN-1 | DEN-3 | |||
| DA4-7 | + | − | − | − | + | − | E | |
| DA6-6 | + | − | − | − | + | − | NS1 | |
| DA6-7 | + | − | − | − | + | − | <1.25 | E |
| DA8-4 | + | − | + | − | + | − | NS1 | |
| DA9-5 | + | + | − | − | + | − | E | |
| DA10-2 | + | − | − | − | + | − | E | |
| DA11-1 | + | − | + | − | + | + | NS1 | |
| DA11-9 | + | − | − | − | + | − | NS1 | |
| DA11-13 | + | − | − | − | + | − | <1.25 | E |
| DA15-2 | + | − | − | − | + | − | NS1 | |
| DA31-6 | + | − | − | − | + | − | NS1 | |
| DA32-9 | + | − | − | − | + | − | NS1 | |
a
E, envelope proteins; NS1, nonstructural protein 1.