Comparison of in vivo and in vitro expression of the plasmid-borne vap genes of R. equi. In vivo expression was determined in lung tissue from five foals experimentally infected with R. equi. In vitro expression was determined during exponential growth of R. equi in broth. Results are presented as means ± SD. Different letters (a, b, and c) indicate a significant difference in expression in vivo between vap genes. Different numbers (1, 2, and 3) indicate a significant difference in expression in vitro between vap genes. *, significant difference in expression of a given vap gene between in vivo and in vitro growth (P < 0.05).