FIG. 1.

Comparison of Leishmania-specific IgG antibodies in different groups of dogs. Antigen-specific antibody was assessed by ELISA using either CSA derived from promastigotes (A) or the rK26 (B), rK39 (C), and rA2 (D) proteins. The recombinant antigens were evaluated for diagnostic potential by using a panel of sera from dogs with proven L. infantum infections grouped by their clinical statuses. Normal control (uninfected) diagnostic samples and cross-reaction control sera were included to achieve adequate precision for estimates of the specificities of the ELISAs. Results are expressed as the optical densities at 492 nm. Dashed lines, cutoff optical densities (mean + 3 standard deviations for normal controls) for each antigen.