TABLE 1.
Sensitivity of ELISAs using crude and recombinant leishmanial antigens for the diagnosis of asymptomatic and symptomatic canine visceral leishmaniasisa
| Antigen | No. of positive sera/no. of tested sera (% sensitivity)b from:
|
Asymptomatic/symptomatic index | |
|---|---|---|---|
| Asymptomatic dogs | Symptomatic dogs | ||
| CSA | 15/50 (30) | 44/50 (88) | 0.34 |
| K26 | 33/50 (66) | 47/50 (94) | 0.70 |
| K39 | 33/50 (66) | 50/50 (100) | 0.66 |
| A2 | 42/48 (88) | 35/50 (70) | 1.2 |
| Totalc | 50/50 (100) | 50/50 (100) | |
a
Infections were proven by parasitological examination (using microscopy, in vitro culture, and/or inoculation of hamsters with biopsy material). At sampling times, dogs were examined for six typical clinical signs of VL and then classified on a semiquantitative scale as described in Materials and Methods.
b
The percent sensitivity was calculated as indicated in Materials and Methods.
c
Dog sera that tested positive with at least one of the recombinant antigens assayed by ELISA.