FIG. 6.

Analysis of the lymphocyte-mediated cytotoxic activity stimulated in vivo in C57BL/6 mice immunized with AdDP as the adjuvant. Spleen cells from naïve C57BL/6 mice labeled with 0.1 μM and 1 μM CFSE were incubated alone or with a peptide encompassing the dominant MHC class I-restricted epitope from OVA (SIINFEKL). After the cells were washed, equal numbers of cells of each type were mixed. A total of 2 × 10⁷ cells were adoptively transferred by intravenous injection into immunized mice. After 16 h, the spleens were harvested and CFSE^hi and CFSE^lo cells were detected by flow cytometry. Results are expressed as the percentage of specific lysis of peptide-pulsed cells. i.n., intranasal.