FIG. 3.

L/D FACS assay for the effects of CM sera on the survival of cells infected with Yersinia. The sera were obtained from animals included in experiment 1 (see Fig. 2). The number in parentheses (n [x axis]) indicates the number of samples. Serum collection times are indicated in the box insert. All assays included control samples of Mφs incubated in the absence of bacteria, Mφs incubated with bacteria alone, and Mφs infected with bacteria pretreated with the protective polyclonal rabbit Abs. The Alhydrogel control CM received adjuvant but no vaccine before challenge. The percent dead was determined as indicated in Materials and Methods; the error bars indicate the standard errors of the mean. The results of statistical analyses of the NHP data are discussed in the text.