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. 2007 Mar 21;14(5):605–616. doi: 10.1128/CVI.00398-06

TABLE 3.

Correlation between results of in vitro assays of sera from mice challenged with Y. pestis strain C12a

Vaccine Challenge dose Correlation variable (assay)c Correlation (P)b
% Livec
Caspase Anti-F1 titer Anti-V titer
FACS Micro
F1+V All Anti-V titer 0.005 NS 0.007 0.010 -
Anti-F1 titer NS NS NS - 0.010
Caspase 0.0004 <0.0001 - NS 0.007
% Live (FACS) - <0.0001 <0.0001 NS 0.005
% Live (Micro) <0.0001 - <0.0001 NS NS
F1-V All Anti-V titer NS 0.048 0.043 NS -
Anti-F1 titer 0.015 0.028 NS - NS
Caspase 0.0002 <0.0001 - NS 0.043
% Live (FACS) - <0.0001 0.0002 0.015 NS
% Live (Micro) <0.0001 - <0.0001 0.028 0.048
V All Anti-V titer NS NS NS NS -
Anti-F1 titer NS NS NS - NS
Caspase <0.0001 <0.0001 - NS NS
% Live (FACS) - <0.0001 <0.0001 NS NS
% Live (Micro) <0.0001 - <0.0001 NS NS
All (combined) 107 Anti-V titer NS NS NS 0.004 -
Anti-F1 titer NS NS NS - 0.004
Caspase 0.0002 0.0002 - NS NS
% Live (FACS) - <0.0001 0.0002 NS NS
% Live (Micro) <0.0001 - 0.0002 NS NS
All 108 Anti-V titer 0.0137 NS NS NS -
Anti-F1 titer NS NS NS - NS
Caspase <0.0001 <0.0001 - NS NS
% Live (FACS) - <0.0001 <0.0001 NS 0.014
% Live (Micro) <0.0001 - <0.0001 NS NS
All 109 Anti-V titer 0.001 0.0031 0.0040 0.001 -
Anti-F1 titer 0.002 NS 0.0050 - 0.001
Caspase 0.001 <0.0001 - 0.005 0.004
% Live (FACS) - <0.0001 0.001 0.002 0.001
% Live (Micro) <0.0001 - <0.0001 NS 0.003
All All (combined) Anti-V titer 0.0002 0.006 0.0004 0.0012 -
Anti-F1 titer 0.003 0.012 NS - 0.001
Caspase <0.0001 <0.0001 - NS 0.0004
% Live (FACS) - <0.0001 <0.0001 0.003 0.0002
% Live (Micro) <0.0001 - <0.0001 0.012 0.006
a

Sera tested were collected prechallenge from a total of 124 mice vaccinated twice with one of three vaccines (V alone, F1-V fusion, or F1+V). The mice were challenged s.c. with Y. pestis C12 (107, 108, or 109 LD50s).

b

Correlations were determined to be significant (P < 0.05) or not significant (NS; P > 0.05) by Pearson product-modified correlation analysis. -, the correlation was not determined or was not applicable.

c

Micro, microscopy. FACS-based and microscopy staining assays were used to determine L/D viability, and the percentages of live cells observed were used for correlation analyses.